Contact an Account ManagerAnalytical methods for the measurement of leukotrienes and other eicosanoids in biological samples from asthmatic subjects
Summary
Type: HPLC
Application #: 118660
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 280 nm
Four HPLC fraction (1 min/fraction, centered around LTE4 standard (E)) were assayed with RIA (details in literature)
Conditions: Eluent: methanol/water/acetic acid, 72:28:0.1, pH adjusted on pH 5.6
Flow rate: 0.8 ml/min
Authors: Kumlin M
Source: Journal of Chromatography A 725 (1996) 29-40
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